Review



primary rabbit polyclonal antibodies against aurka  (Proteintech)


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    Structured Review

    Proteintech primary rabbit polyclonal antibodies against aurka
    A. The protein expression of Ki-67, <t>AURKA</t> and NDC80 was significantly increased with increasing OD grades, and semiquantitative analysis was performed in 5 NBTs, 57 OIIs and 29 OIIIs; B. Kaplan–Meier survival analysis showed that high expression of these three proteins indicated a poor survival. The columns represent the same samples; 511 and 1278 refer to the sample ID in the CGGA dataset.
    Primary Rabbit Polyclonal Antibodies Against Aurka, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+rabbit+polyclonal+antibodies+against+aurka/Aurora+A+Antibody/pmc04741997-172-0-6
    Average 93 stars, based on 24 article reviews
    primary rabbit polyclonal antibodies against aurka - by Bioz Stars, 2026-10
    93/100 stars

    Images

    1) Product Images from "Co-expression of mitosis-regulating genes contributes to malignant progression and prognosis in oligodendrogliomas"

    Article Title: Co-expression of mitosis-regulating genes contributes to malignant progression and prognosis in oligodendrogliomas

    Journal: Oncotarget

    doi:

    A. The protein expression of Ki-67, AURKA and NDC80 was significantly increased with increasing OD grades, and semiquantitative analysis was performed in 5 NBTs, 57 OIIs and 29 OIIIs; B. Kaplan–Meier survival analysis showed that high expression of these three proteins indicated a poor survival. The columns represent the same samples; 511 and 1278 refer to the sample ID in the CGGA dataset.
    Figure Legend Snippet: A. The protein expression of Ki-67, AURKA and NDC80 was significantly increased with increasing OD grades, and semiquantitative analysis was performed in 5 NBTs, 57 OIIs and 29 OIIIs; B. Kaplan–Meier survival analysis showed that high expression of these three proteins indicated a poor survival. The columns represent the same samples; 511 and 1278 refer to the sample ID in the CGGA dataset.

    Techniques Used: Expressing

    A. The three proteins (Ki-67, AURKA and NDC80) showed similar expression levels and significantly increased with increasing tumor grades; B. Western blotting showed that suppression of the expression of AURKA and NDC80 with siRNAs significantly decreased their protein expression; C. and D. MTT assay and cell immunofluorescence showed that suppression of AURKA and NDC80 expression suppressed the malignant proliferation of glioma cells and Ki-67 expression. Parental cells are non-transfected H4 and LN229 cells; Negative Control refers to transfected H4 and LN229 cells with a scrambled siRNA sequence that will not lead to specific degradation of any known cellular mRNA.
    Figure Legend Snippet: A. The three proteins (Ki-67, AURKA and NDC80) showed similar expression levels and significantly increased with increasing tumor grades; B. Western blotting showed that suppression of the expression of AURKA and NDC80 with siRNAs significantly decreased their protein expression; C. and D. MTT assay and cell immunofluorescence showed that suppression of AURKA and NDC80 expression suppressed the malignant proliferation of glioma cells and Ki-67 expression. Parental cells are non-transfected H4 and LN229 cells; Negative Control refers to transfected H4 and LN229 cells with a scrambled siRNA sequence that will not lead to specific degradation of any known cellular mRNA.

    Techniques Used: Expressing, Western Blot, MTT Assay, Immunofluorescence, Transfection, Negative Control, Sequencing

    Related Articles

    Expressing:

    Article Title: Co-expression of mitosis-regulating genes contributes to malignant progression and prognosis in oligodendrogliomas
    Article Snippet: Equal amounts of total protein (30 μg) from the cell lysates were loaded onto a 10% SDS/PAGE gel, transferred to a PVDF membrane (Millipore) and detected using an ECL western blotting Detection System (Biorad).Equal amounts of total protein (30 μg) from the cell lysates were loaded onto a 10% SDS/PAGE gel, transferred to a PVDF membrane (Millipore) and detected using an ECL western blotting Detection System (Biorad).. Primary rabbit polyclonal antibodies against AURKA (Proteintech Group, Inc., Catalog No.: 10297–1-AP, 1:200) and NDC80 (Proteintech Group, Inc., Catalog No.: 18932–1-AP, 1:500) were used.. An antibody against GAPDH (Proteintech Group, Inc., Catalog No.: 60004–1-Ig, 1:5000) was used as the loading control.An antibody against GAPDH (Proteintech Group, Inc., Catalog No.: 60004–1-Ig, 1:5000) was used as the loading control.

    Western Blot:

    Article Title: Co-expression of mitosis-regulating genes contributes to malignant progression and prognosis in oligodendrogliomas
    Article Snippet: Equal amounts of total protein (30 μg) from the cell lysates were loaded onto a 10% SDS/PAGE gel, transferred to a PVDF membrane (Millipore) and detected using an ECL western blotting Detection System (Biorad).Equal amounts of total protein (30 μg) from the cell lysates were loaded onto a 10% SDS/PAGE gel, transferred to a PVDF membrane (Millipore) and detected using an ECL western blotting Detection System (Biorad).. Primary rabbit polyclonal antibodies against AURKA (Proteintech Group, Inc., Catalog No.: 10297–1-AP, 1:200) and NDC80 (Proteintech Group, Inc., Catalog No.: 18932–1-AP, 1:500) were used.. An antibody against GAPDH (Proteintech Group, Inc., Catalog No.: 60004–1-Ig, 1:5000) was used as the loading control.An antibody against GAPDH (Proteintech Group, Inc., Catalog No.: 60004–1-Ig, 1:5000) was used as the loading control.

    MTT Assay:

    Article Title: Co-expression of mitosis-regulating genes contributes to malignant progression and prognosis in oligodendrogliomas
    Article Snippet: Equal amounts of total protein (30 μg) from the cell lysates were loaded onto a 10% SDS/PAGE gel, transferred to a PVDF membrane (Millipore) and detected using an ECL western blotting Detection System (Biorad).Equal amounts of total protein (30 μg) from the cell lysates were loaded onto a 10% SDS/PAGE gel, transferred to a PVDF membrane (Millipore) and detected using an ECL western blotting Detection System (Biorad).. Primary rabbit polyclonal antibodies against AURKA (Proteintech Group, Inc., Catalog No.: 10297–1-AP, 1:200) and NDC80 (Proteintech Group, Inc., Catalog No.: 18932–1-AP, 1:500) were used.. An antibody against GAPDH (Proteintech Group, Inc., Catalog No.: 60004–1-Ig, 1:5000) was used as the loading control.An antibody against GAPDH (Proteintech Group, Inc., Catalog No.: 60004–1-Ig, 1:5000) was used as the loading control.

    Immunofluorescence:

    Article Title: Co-expression of mitosis-regulating genes contributes to malignant progression and prognosis in oligodendrogliomas
    Article Snippet: Equal amounts of total protein (30 μg) from the cell lysates were loaded onto a 10% SDS/PAGE gel, transferred to a PVDF membrane (Millipore) and detected using an ECL western blotting Detection System (Biorad).Equal amounts of total protein (30 μg) from the cell lysates were loaded onto a 10% SDS/PAGE gel, transferred to a PVDF membrane (Millipore) and detected using an ECL western blotting Detection System (Biorad).. Primary rabbit polyclonal antibodies against AURKA (Proteintech Group, Inc., Catalog No.: 10297–1-AP, 1:200) and NDC80 (Proteintech Group, Inc., Catalog No.: 18932–1-AP, 1:500) were used.. An antibody against GAPDH (Proteintech Group, Inc., Catalog No.: 60004–1-Ig, 1:5000) was used as the loading control.An antibody against GAPDH (Proteintech Group, Inc., Catalog No.: 60004–1-Ig, 1:5000) was used as the loading control.

    Transfection:

    Article Title: Co-expression of mitosis-regulating genes contributes to malignant progression and prognosis in oligodendrogliomas
    Article Snippet: Equal amounts of total protein (30 μg) from the cell lysates were loaded onto a 10% SDS/PAGE gel, transferred to a PVDF membrane (Millipore) and detected using an ECL western blotting Detection System (Biorad).Equal amounts of total protein (30 μg) from the cell lysates were loaded onto a 10% SDS/PAGE gel, transferred to a PVDF membrane (Millipore) and detected using an ECL western blotting Detection System (Biorad).. Primary rabbit polyclonal antibodies against AURKA (Proteintech Group, Inc., Catalog No.: 10297–1-AP, 1:200) and NDC80 (Proteintech Group, Inc., Catalog No.: 18932–1-AP, 1:500) were used.. An antibody against GAPDH (Proteintech Group, Inc., Catalog No.: 60004–1-Ig, 1:5000) was used as the loading control.An antibody against GAPDH (Proteintech Group, Inc., Catalog No.: 60004–1-Ig, 1:5000) was used as the loading control.

    Negative Control:

    Article Title: Co-expression of mitosis-regulating genes contributes to malignant progression and prognosis in oligodendrogliomas
    Article Snippet: Equal amounts of total protein (30 μg) from the cell lysates were loaded onto a 10% SDS/PAGE gel, transferred to a PVDF membrane (Millipore) and detected using an ECL western blotting Detection System (Biorad).Equal amounts of total protein (30 μg) from the cell lysates were loaded onto a 10% SDS/PAGE gel, transferred to a PVDF membrane (Millipore) and detected using an ECL western blotting Detection System (Biorad).. Primary rabbit polyclonal antibodies against AURKA (Proteintech Group, Inc., Catalog No.: 10297–1-AP, 1:200) and NDC80 (Proteintech Group, Inc., Catalog No.: 18932–1-AP, 1:500) were used.. An antibody against GAPDH (Proteintech Group, Inc., Catalog No.: 60004–1-Ig, 1:5000) was used as the loading control.An antibody against GAPDH (Proteintech Group, Inc., Catalog No.: 60004–1-Ig, 1:5000) was used as the loading control.

    Sequencing:

    Article Title: Co-expression of mitosis-regulating genes contributes to malignant progression and prognosis in oligodendrogliomas
    Article Snippet: Equal amounts of total protein (30 μg) from the cell lysates were loaded onto a 10% SDS/PAGE gel, transferred to a PVDF membrane (Millipore) and detected using an ECL western blotting Detection System (Biorad).Equal amounts of total protein (30 μg) from the cell lysates were loaded onto a 10% SDS/PAGE gel, transferred to a PVDF membrane (Millipore) and detected using an ECL western blotting Detection System (Biorad).. Primary rabbit polyclonal antibodies against AURKA (Proteintech Group, Inc., Catalog No.: 10297–1-AP, 1:200) and NDC80 (Proteintech Group, Inc., Catalog No.: 18932–1-AP, 1:500) were used.. An antibody against GAPDH (Proteintech Group, Inc., Catalog No.: 60004–1-Ig, 1:5000) was used as the loading control.An antibody against GAPDH (Proteintech Group, Inc., Catalog No.: 60004–1-Ig, 1:5000) was used as the loading control.



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    Proteintech primary rabbit polyclonal antibodies against aurka
    A. The protein expression of Ki-67, <t>AURKA</t> and NDC80 was significantly increased with increasing OD grades, and semiquantitative analysis was performed in 5 NBTs, 57 OIIs and 29 OIIIs; B. Kaplan–Meier survival analysis showed that high expression of these three proteins indicated a poor survival. The columns represent the same samples; 511 and 1278 refer to the sample ID in the CGGA dataset.
    Primary Rabbit Polyclonal Antibodies Against Aurka, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+rabbit+polyclonal+antibodies+against+aurka/Aurora+A+Antibody/pmc04741997-172-0-6
    Average 93 stars, based on 1 article reviews
    primary rabbit polyclonal antibodies against aurka - by Bioz Stars, 2026-10
    93/100 stars
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    A. The protein expression of Ki-67, AURKA and NDC80 was significantly increased with increasing OD grades, and semiquantitative analysis was performed in 5 NBTs, 57 OIIs and 29 OIIIs; B. Kaplan–Meier survival analysis showed that high expression of these three proteins indicated a poor survival. The columns represent the same samples; 511 and 1278 refer to the sample ID in the CGGA dataset.

    Journal: Oncotarget

    Article Title: Co-expression of mitosis-regulating genes contributes to malignant progression and prognosis in oligodendrogliomas

    doi:

    Figure Lengend Snippet: A. The protein expression of Ki-67, AURKA and NDC80 was significantly increased with increasing OD grades, and semiquantitative analysis was performed in 5 NBTs, 57 OIIs and 29 OIIIs; B. Kaplan–Meier survival analysis showed that high expression of these three proteins indicated a poor survival. The columns represent the same samples; 511 and 1278 refer to the sample ID in the CGGA dataset.

    Article Snippet: Primary rabbit polyclonal antibodies against AURKA (Proteintech Group, Inc., Catalog No.: 10297–1-AP, 1:200) and NDC80 (Proteintech Group, Inc., Catalog No.: 18932–1-AP, 1:500) were used.

    Techniques: Expressing

    A. The three proteins (Ki-67, AURKA and NDC80) showed similar expression levels and significantly increased with increasing tumor grades; B. Western blotting showed that suppression of the expression of AURKA and NDC80 with siRNAs significantly decreased their protein expression; C. and D. MTT assay and cell immunofluorescence showed that suppression of AURKA and NDC80 expression suppressed the malignant proliferation of glioma cells and Ki-67 expression. Parental cells are non-transfected H4 and LN229 cells; Negative Control refers to transfected H4 and LN229 cells with a scrambled siRNA sequence that will not lead to specific degradation of any known cellular mRNA.

    Journal: Oncotarget

    Article Title: Co-expression of mitosis-regulating genes contributes to malignant progression and prognosis in oligodendrogliomas

    doi:

    Figure Lengend Snippet: A. The three proteins (Ki-67, AURKA and NDC80) showed similar expression levels and significantly increased with increasing tumor grades; B. Western blotting showed that suppression of the expression of AURKA and NDC80 with siRNAs significantly decreased their protein expression; C. and D. MTT assay and cell immunofluorescence showed that suppression of AURKA and NDC80 expression suppressed the malignant proliferation of glioma cells and Ki-67 expression. Parental cells are non-transfected H4 and LN229 cells; Negative Control refers to transfected H4 and LN229 cells with a scrambled siRNA sequence that will not lead to specific degradation of any known cellular mRNA.

    Article Snippet: Primary rabbit polyclonal antibodies against AURKA (Proteintech Group, Inc., Catalog No.: 10297–1-AP, 1:200) and NDC80 (Proteintech Group, Inc., Catalog No.: 18932–1-AP, 1:500) were used.

    Techniques: Expressing, Western Blot, MTT Assay, Immunofluorescence, Transfection, Negative Control, Sequencing