primary rabbit polyclonal antibodies against aurka (Proteintech)
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Primary Rabbit Polyclonal Antibodies Against Aurka, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+rabbit+polyclonal+antibodies+against+aurka/Aurora+A+Antibody/pmc04741997-172-0-6
Average 93 stars, based on 24 article reviews
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1) Product Images from "Co-expression of mitosis-regulating genes contributes to malignant progression and prognosis in oligodendrogliomas"
Article Title: Co-expression of mitosis-regulating genes contributes to malignant progression and prognosis in oligodendrogliomas
Journal: Oncotarget
doi:
Figure Legend Snippet: A. The protein expression of Ki-67, AURKA and NDC80 was significantly increased with increasing OD grades, and semiquantitative analysis was performed in 5 NBTs, 57 OIIs and 29 OIIIs; B. Kaplan–Meier survival analysis showed that high expression of these three proteins indicated a poor survival. The columns represent the same samples; 511 and 1278 refer to the sample ID in the CGGA dataset.
Techniques Used: Expressing
Figure Legend Snippet: A. The three proteins (Ki-67, AURKA and NDC80) showed similar expression levels and significantly increased with increasing tumor grades; B. Western blotting showed that suppression of the expression of AURKA and NDC80 with siRNAs significantly decreased their protein expression; C. and D. MTT assay and cell immunofluorescence showed that suppression of AURKA and NDC80 expression suppressed the malignant proliferation of glioma cells and Ki-67 expression. Parental cells are non-transfected H4 and LN229 cells; Negative Control refers to transfected H4 and LN229 cells with a scrambled siRNA sequence that will not lead to specific degradation of any known cellular mRNA.
Techniques Used: Expressing, Western Blot, MTT Assay, Immunofluorescence, Transfection, Negative Control, Sequencing
Related Articles
Expressing:Article Title: Co-expression of mitosis-regulating genes contributes to malignant progression and prognosis in oligodendrogliomas Article Snippet: Equal amounts of total protein (30 μg) from the cell lysates were loaded onto a 10% SDS/PAGE gel, transferred to a PVDF membrane (Millipore) and detected using an ECL western blotting Detection System (Biorad).Equal amounts of total protein (30 μg) from the cell lysates were loaded onto a 10% SDS/PAGE gel, transferred to a PVDF membrane (Millipore) and detected using an ECL western blotting Detection System (Biorad).. Western Blot:Article Title: Co-expression of mitosis-regulating genes contributes to malignant progression and prognosis in oligodendrogliomas Article Snippet: Equal amounts of total protein (30 μg) from the cell lysates were loaded onto a 10% SDS/PAGE gel, transferred to a PVDF membrane (Millipore) and detected using an ECL western blotting Detection System (Biorad).Equal amounts of total protein (30 μg) from the cell lysates were loaded onto a 10% SDS/PAGE gel, transferred to a PVDF membrane (Millipore) and detected using an ECL western blotting Detection System (Biorad).. MTT Assay:Article Title: Co-expression of mitosis-regulating genes contributes to malignant progression and prognosis in oligodendrogliomas Article Snippet: Equal amounts of total protein (30 μg) from the cell lysates were loaded onto a 10% SDS/PAGE gel, transferred to a PVDF membrane (Millipore) and detected using an ECL western blotting Detection System (Biorad).Equal amounts of total protein (30 μg) from the cell lysates were loaded onto a 10% SDS/PAGE gel, transferred to a PVDF membrane (Millipore) and detected using an ECL western blotting Detection System (Biorad).. Immunofluorescence:Article Title: Co-expression of mitosis-regulating genes contributes to malignant progression and prognosis in oligodendrogliomas Article Snippet: Equal amounts of total protein (30 μg) from the cell lysates were loaded onto a 10% SDS/PAGE gel, transferred to a PVDF membrane (Millipore) and detected using an ECL western blotting Detection System (Biorad).Equal amounts of total protein (30 μg) from the cell lysates were loaded onto a 10% SDS/PAGE gel, transferred to a PVDF membrane (Millipore) and detected using an ECL western blotting Detection System (Biorad).. Transfection:Article Title: Co-expression of mitosis-regulating genes contributes to malignant progression and prognosis in oligodendrogliomas Article Snippet: Equal amounts of total protein (30 μg) from the cell lysates were loaded onto a 10% SDS/PAGE gel, transferred to a PVDF membrane (Millipore) and detected using an ECL western blotting Detection System (Biorad).Equal amounts of total protein (30 μg) from the cell lysates were loaded onto a 10% SDS/PAGE gel, transferred to a PVDF membrane (Millipore) and detected using an ECL western blotting Detection System (Biorad).. Negative Control:Article Title: Co-expression of mitosis-regulating genes contributes to malignant progression and prognosis in oligodendrogliomas Article Snippet: Equal amounts of total protein (30 μg) from the cell lysates were loaded onto a 10% SDS/PAGE gel, transferred to a PVDF membrane (Millipore) and detected using an ECL western blotting Detection System (Biorad).Equal amounts of total protein (30 μg) from the cell lysates were loaded onto a 10% SDS/PAGE gel, transferred to a PVDF membrane (Millipore) and detected using an ECL western blotting Detection System (Biorad).. Sequencing:Article Title: Co-expression of mitosis-regulating genes contributes to malignant progression and prognosis in oligodendrogliomas Article Snippet: Equal amounts of total protein (30 μg) from the cell lysates were loaded onto a 10% SDS/PAGE gel, transferred to a PVDF membrane (Millipore) and detected using an ECL western blotting Detection System (Biorad).Equal amounts of total protein (30 μg) from the cell lysates were loaded onto a 10% SDS/PAGE gel, transferred to a PVDF membrane (Millipore) and detected using an ECL western blotting Detection System (Biorad).. |